Tylenol's Impact on Glutathione and Liver Damage Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research Let's talk Tylenol Paracetamol (acetaminophen) There are many side effects that most people don't realize. OTC does not mean it is side effect free, especially with chronic use. Even just 4 days of Electrochemical evidences in oxidation of acetaminophen in the presence of glutathione and N acetylcysteine Chemical Communications (RSC Publishing) Glutathione, Alcohol and drugs