Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health HA Rejuve: Micro Injected Glutathione & HA Can Remove Years of Sun Damage Before & After Photos New Jersey Reflections Center Acetaminophen toxicity EMCrit Project 5 Oxoprolinuria as a Cause for Metabolic Acidosis Renal Fellow Network A guide to managing acute liver failure Cleveland Clinic Journal of Medicine