Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Metabolism of acetaminophen (paracetamol) in plantstwo independent pathways result in the formation of a glutathione and a glucose conjugate Environmental Science and Pollution Research Springer Nature Link As a naturopathic doctor and a mom, Tylenol is a hard no in my house and I want to explain why. , , Acetaminophen (Tylenol) is processed through the liver, and in that process it can lower Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: Sex differences in glutathione metabolism and acetaminophen toxicity Taking Tylenol? The Glutathione Connection Dr. Green Mom