Frontiers Herbal Therapy for the Treatment of Acetaminophen Associated Liver Injury: Recent Advances and Future Perspectives 1. Widespread Use of Acetaminophen ATrain Education Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Frontiers Liquid Chromatography Tandem Mass Spectrometry Analysis of Acetaminophen Covalent Binding to Glutathione S Transferases Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research