Tylenol's Impact on Glutathione and Liver Damage Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen metabolism. Acetaminophen is metabolized primarily by Download Scientific Diagram Sulforaphane Benefits for Hormone Balance and Health Acetaminophen (Paracetamol) Springer Nature Link Transcription Factor Nrf2 Is Essential for Induction of NAD(P)H:Quinone Oxidoreductase 1, Glutathione S Transferases, and Glutamate Cysteine Ligase by Broccoli Seeds and Isothiocyanates The Journal of Nutrition