Acetaminophen Toxicity in Children: Diagnosis, Clinical Assessment, and Treatment of Acute Overingestion Consultant360 Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Acetaminophen at low doses depletes airway glutathione and alters respiratory reflex responses Smith 2013 The FASEB Journal Wiley Online Library Can I give Tylenol (acetaminophen) to a patient with elevated aspartate aminotransferase (AST) alanine aminotransferase (ALT) levels? Sex differences in glutathione metabolism and acetaminophen toxicity