A Possible Antidote for Tylenol (Paracetamol) Overdose American Council on Science and Health Tylenol and glutathione, actually explainedhere's the bullet points: * Lots of concern raised on social media re: glutathione depletion and acetaminophen use * Glutathione is used in the metabolism View of Acetaminophen, the missing anion gap in the patient with metabolic acidosis The Southwest journal of medicine Acetaminophen Pathway (toxic doses), Pharmacokinetics Acetaminophen Depletes Glutathione Essential Formulas Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.