Acetaminophen Depletes Glutathione Essential Formulas The Hidden Impact of Acetaminophen Well Rooted Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol's Impact on Glutathione and Liver Damage Why You Need To Avoid Tylenol Before and After Vaccines Glutathione, Tylenol & Your Health: What You Should Know